HPLC Column Maintenance
110 years ago, when Russian botanist Tswett studied the pigment components of plant leaves, he poured the extract of plant leaves into an upright glass tube filled with calcium carbonate, and then added petroleum ether to allow it to flow freely. Separated from each other to form bands of different colors. This method is called “chromatography” and the glass tube used is called “column”.
In recent years, thanks to strong demand in the fields of life sciences, food safety, and environmental protection, the global column market has grown rapidly, and the three major markets in North America, Europe, and Asia continue to expand.
So how do we maintain the HPLC column? First of all, before the column is used, it is best to perform the performance test of the HPLC column as a reference for evaluating the performance change of the column in the future. The reversed phase column was factory tested and stored in methanol (acetonitrile) / water. The new column should first be washed with 10-20 column volumes of methanol or acetonitrile.
Post-laboratory maintenance: Use buffer or saline mobile phase. After the experiment is completed, rinse with 10% methanol/water for 30 minutes, wash off the salt in the column, and rinse with methanol for 30 minutes. Note: The column should not be rinsed with pure water. 10% methanol should be added to the water to prevent the packing from collapsing.
Strengthening the maintenance of the HPLC column is of great significance for the application of high-pressure liquid chromatography in the column. The standard operating procedures for HPLC column management are established and strictly implemented. Each newly purchased column must be registered and registered, including: production company, product name, model, specification, purchase time, activation time, unit price, and column protection solution. The column is classified and stored. The column of silica gel, chemically bonded silica gel, cyano or amino column, ion exchange resin column and gel column are each labeled with a column, and the label is marked on the box.
A liquid chromatograph refers to an instrument that first separates the mixture and then analyzes and identifies it by using the difference in the distribution ratio between the liquid-solid or immiscible liquids. In order to ensure the normal operation of the instrument, the daily maintenance work of the column is indispensable.