Rational Handling of SPE Cartridge
Adsorbents in the SPE column may have a small amount of interferents, effective activation can avoid these interferents entering the eluent, the activation solution should select the solvent system with strong elution strength throughout the operation; in reverse phase mode, from the sample solution.
The eluent is mainly related to 0%-100% aqueous methanol solution, so the activation solution should be pure methanol.
When the target compound needs to be eluted with a methanol-methyl tert-butyl ether mixture, the activation solution should be selected by methyl tert-butyl. Ether;
In the reversed phase mode, from the sample solution to the eluent is mainly involved 0%-100% hexane-methyl tert-butyl ether solution, so the activation solution should be pure methyl t-butyl ether.
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