Requirements of Liquid Chromatograph for Surrounding Environment
Like most analytical instruments, liquid chromatograph has certain requirements for the environment around the instrument, but there are not too special requirements to meet the basic conditions of the general analytical instrument room, which can ensure the stable operation and safe operation of the instrument.
Indoor lighting should be light enough to avoid direct sunlight. To avoid the influence of direct light on instrument operation and observation, the instrument (display) should be laterally lit. Curtain should be hung in laboratory windows to adjust light intensity at any time.
In the process of analysis by liquid chromatography, the volatile organic solvents (methanol, acetonitrile, etc.) in the mobile phase will produce harmful gases, which will corrode the optical components of the instrument. The ventilation in the instrument room should be paid attention to remove harmful gases and keep the air fresh.
There are two main ways of ventilation: one is natural ventilation, regularly open doors and windows, so that the internal and external air convection; the other is forced ventilation, such as the use of ventilators, exhaust fans, timely discharge of harmful gases outside.
(3) ambient temperature and humidity:
Suitable temperature and humidity are necessary conditions to ensure normal operation and stable performance of HPLC instrument.
The general requirement of the HPLC is that the room temperature should be between 20-30 C and the daily temperature should be 2-3 C. The fluctuation should not be too large, otherwise, the chromatographic separation will be affected when the analysis is started.
In summer, we must control the room temperature not to exceed 30 degrees Celsius, too high temperature will cause bubbles in the flow system, affecting the normal operation of the detector (such as fluorescent detector). Indoor relative humidity should be controlled below 60%.
(4) power supply:
The liquid chromatography chamber should be equipped with lighting power and power supply, and power electric equipment can be used for the operation of the instrument. In order to reduce noise interference, the instrument should be far away from instantaneous power supply of high-power electrical equipment, such as: large-scale electric equipment (ultra-low temperature refrigerators, electric ovens, etc.), elevators, air conditioning systems. In order to ensure the accuracy of the infusion pump of HPLC, it is necessary to ensure a stable input voltage. Therefore, it is suggested that the chromatographic chamber should be equipped with a stable power supply equivalent to the instrument load. Indoor walls should be equipped with grounding resistance to meet the requirements of the ground wire, for instrument and equipment protective grounding of the common wire grounding resistance <4_.
Particulate matter suspended in the air will interfere with chromatographic instrument analysis, make background and noise rise, become a source of pollution for analysis and testing, directly affect the purity of high-purity substances and trace impurities analysis.
HPLC instruments are desktop-type. Detectors and optical system are enclosed in the instruments. They are less disturbed by the dust in the ground and air, and generally do not need buffer separation. However, attention should also be paid to keeping indoor cleanliness, especially when the instruments are placed on the first floor, because the dust in the air is large, in order to intercept the sources of particulate pollution, the ground should be cleaned frequently. The surface and the tabletop do not accumulate dust or dust.