Selection of SPE Cartridges and Filter Membranes in Solid Phase Extraction
Solid phase extraction is a sample pretreatment instrument. Using the liquid-solid phase chromatography theory, the sample is enriched, separated and purified by selective adsorption and elution to make the sample purer, thus reducing the impurity in the sample. The detected interference greatly improves the accuracy of the detection.
The principle of solid phase extraction is the separation principle using liquid chromatography with selective adsorption and selective elution. The more common method is to pass the liquid sample solution through the adsorbent, retain the substance to be tested, and then use the appropriate strength solvent to wash away the impurities, and then quickly elute the test substance with a small amount of solvent, thereby achieving the purpose of rapid separation, purification and concentration. Solid phase extraction can also be viewed as a simple chromatographic separation process.
How to choose SPE cartridge and filter membrane in solid phase extraction?
The size and specifications of the SPE cartridge and filter membrane should depend on the concentration of the analyte in the sample. For the lower concentration in vivo samples, it is generally preferred to use a minimum of stationary phase filler to extract larger volumes of sample.