The Silane Process of the Automatic Sample Bottle
Silanization is the process of surface treatment of metal or nonmetallic materials by organic silane solution.
Principle: glass surface silanol groups (Si – OH), can produce the adsorption of proteins such as samples, thereby causing loss to the samples. The class groups available silanization closed processing method, through the silicon alkylation reagents dimethyldichlorosilane or six methyl two silicon amine and carrier surface reaction, hydrogen bonding ability, remove the class groups, to improve the performance on the surface of the carrier, to reduce the adsorption of the sample. In dealing with low concentration of special “sticky” solutes, such as single nucleic acids or proteins are especially important.
Advantage: the sample bottle reduces the polarity of the silane treated samples in glass wall adsorption, some compounds, such as amino acid, protein or phenolic compounds, even the chromatographic analysis is produced with level 1 hydrolytic glass material also have varying degrees of adsorption, silane treatment is the deactivation of the surface of the glass, in order to eliminate the polarity between the sample and glass.