The Sorbent of HPLC Column
In the liquid chromatographic separation system, whether the packing material is selected correctly or not will directly affect the quality of the separation material of the professional liquid chromatographic column. Since the liquid chromatography packing has a wide selection range, it is necessary for us to have a simple understanding and understanding of the different liquid chromatography packing before buying liquid chromatography column.
First, silica gel matrix filler in positive phase chromatography. The characteristic of substance separation is that the weakly weak combination is first washed out by the chromatographic column.
Second, reverse phase chromatography silica gel matrix filler. The characteristic of substance separation is that the stronger polar combination is first washed out by the chromatographic column, while the weakly weak component will be stronger on the column.
Third, polymer filler. It is characterized by a wide range of pH and a strong hydrophobicity, but its column efficiency is lower than that of silica matrix.
All in all, if we can have a comprehensive and systematic understanding of the classification and characteristics of liquid column packing, we can save the time to choose the purchase.