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Two Common Types of HPLC

Liquid-liquid Partition Chromatography

The mobile phase and the stationary phase both are liquid. The mobile phase and the stationary phase should be immiscible (different polarity, just to avoid the loss of fixation solution).

Normal Phase liquid Chromatography: The polarity of the mobile phase is less than the polarity of the stationary solution.

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Reverse Phase liquid Chromatography: The polarity of the mobile phase is greater than the polarity of the stationary solution.

The disadvantages of Liquid-liquid Partition Chromatography: Although the polarity requirements of the mobile phase and the stationary phase are completely different, the fixed liquid in the mobile phase is still slightly dissolved; when mobile phase through the column, the mechanical impact force will cause the loss of stationary solution. But Chemically Bonded Phase Chromatography can overcome the above shortcomings.
Liquid-solid chromatography

The mobile phase is liquid, the stationary phase is adsorbent (such as silica gel, alumina, etc.). They were separated based on the different adsorption of substances. The mechanism of action is that when the sample enters the column, the solute molecule (X) and the solvent molecule (S) compete with the active center adsorbent surface.

2018-06-15T08:37:58+00:00 October 31st, 2017|